LACDR microscopes
Nikon 2
Location: GW.2.26a
Nikon 2 is a fully automated inverted confocal microscope best suited for fixed fluorescent samples and small duration live cell imaging (e.g 24 hour timelapse).
Hardware
Microscope model: Inverted Nikon Ti eclipse
Stage: Nikon, encoded
Confocal scanner modalities: Nikon A1 Galvano, single and bidirectional.
Incubation: Custom made incubator with temperature and CO2 control
Detectors: four PMTs
Lasers and filters
Laser lines: 405nm, 488nm. 561nm, 640nm
Filters available: 450/50, 482/35, 525/50, 540/30, 515/30, 595/50, 585/65, 700/75
Software/Automation
Software: NiS elements 5.02.03
Automation: Jobs & ND acquisition
GA version: (TBD)
Nikon 3
Location: GW.2.30
Nikon 3 is a fully automated inverted confocal microscope capable of fast imaging (up to 30fps in resonant mode) and fast Z-stacking due to the Piezo electric stage insert. The additional transmission detection can be used to take pseudo brightfield images. It also has more sensitive detectors for the green and red channels (channels 2 and 3). Also capable for longer live cell imaging sessions (up to 3 days)
Hardware
Microscope model: Inverted Nikon Ti eclipse
Stage: Nikon, encoded with MCL nano drive Piezo insert
Confocal scanner modalities: Nikon A1R MP Galvano and resonant, single and bidirectional.
Incubation: OKO lab incubator with temperature and CO2 control settings
Detectors: Channel 1 and 4: PMT, channels 2 and 3: GaAsP. 1 transmission detector
Lasers and filters
Laser lines: 405nm, 488nm. 561nm, 640nm
Filters available: 450/50, 482/35, 450/50 Ircut, 525/50, 540/30, 515/30, 585/50, 585/65, 700/75
Software/Automation
Software: NiS elements 4.5
Automation: Jobs & ND acquisition
GA version: GA1
Nikon 4
Location: GW.2.24
Nikon 4 is a fully automated inverted confocal microscope, the tuneable detector provides flexibility on the detection bandwidth albeit at the cost of overall imaging speed. The OKO lab incubator provides O2 control allowing for hypoxic conditions and it is well suited for longer timelapse experiments (up to three days).
Hardware
Microscope model: Inverted Nikon Ti2 eclipse
Stage: Nikon, encoded.
Confocal scanner modalities: Nikon C2Si Galvano single and bidirectional.
Incubation: OKO lab incubator with temperature and CO2 control and O2 settings for hypoxic conditions.
Detectors: Channel 1 GaAsP spectral, Channel 2 GaAsP fluorescence
Lasers and filters
Laser lines: 405nm, 488nm. 561nm, 640nm
Filters available: The C2Si has a single tuneable detector for fluorescence allowing for effectively any bandwidth filter from 400nm to 720nm.
Software/Automation
Software: NiS elements 5.11.03
Automation: Jobs & ND acquisition
GA version: (TBD)